Amplified fluorescence polarization aptasensors based on structure-switching-triggered nanoparticles enhancement for bioassays.

نویسندگان

  • Yong Huang
  • Shulin Zhao
  • Zhen-Feng Chen
  • Ming Shi
  • Hong Liang
چکیده

We have developed an amplified fluorescence polarization aptasensor that relies on aptamer structure-switching-triggered nanoparticles (NPs) enhancement for biomolecules detection. This new type of assay exhibits higher detection sensitivity over traditional homogeneous aptasensors by two orders of magnitude and high specificity for target molecules.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Colorimetric gold nanoparticles-based aptasensors

Recognition of different agents including chemical and biological plays important role in forensic, biomedical and environmentalfield.In recent decades, nanotechnology and nano materials had a high impact on development of sensors. Using  nanomaterials in construction of biosensors can effectively improve the Sensitivity and other features of biosensors. Different type of nanostructures includi...

متن کامل

Use of laser-triggered gold nanoparticle-grafted dual light and temperature-responsive polymeric sensor for the recognition of thioguanine as anti-tumor agent

Objective(s): Today, there is an urgent need for improved sensor materials for drug sensing and effective monitoring and interventions in this area are highly required to struggle drug abuse. The present study aimed to synthesize a thioguanine-responsive sensor based on a nanocomposite consisting of AuNP-grafted light- and temperature-responsive poly butylmethacrylate-co-acrylamide-co-methacryl...

متن کامل

Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization.

We have developed a highly sensitive and selective fluorescence polarization assay method based on the specificity of the DNA cleavage reaction with the enhancement of gold nanoparticles (AuNPs) for assaying endonuclease activity and inhibition. This assay can detect EcoRI endonuclease down to 5.0×10(-4) U mL(-1) with a detection range from 5.0×10(-4) to 10 U mL(-1).

متن کامل

Enzyme-free fluorescent-amplified aptasensors based on target-responsive DNA strand displacement via toehold-mediated click chemical ligation.

A new target-responsive DNA strand displacement system via toehold-mediated click chemical ligation was designed and prepared for enzyme-free fluorescent-amplified aptasensors. The aptasensors significantly amplified fluorescent signals in response to targets based on target recycling processes.

متن کامل

Successively amplified electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement.

A successively signal-amplified electrochemical immunoassay has been reported on the basis of the biocatalytic deposition of silver nanoparticles with their subsequent enlargement by nanoparticle-promoted catalytic precipitation of silver from the silver-enhancer solution. The immunoassay was carried out based on a heterogeneous sandwich procedure using polystyrene microwells to immobilize anti...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Chemical communications

دوره 48 60  شماره 

صفحات  -

تاریخ انتشار 2012